goat nonimmune igg control antibody  (Vector Laboratories)

Journal: Biology of Reproduction

Article Title: Extraembryonic heparin-binding epidermal growth factor-like growth factor deficiency compromises placentation in mice †

doi: 10.1093/biolre/ioy174

Figure Lengend Snippet: The effect of Hbegf genotype on expression of VEGF in the mouse placenta. Immunohistochemical labeling with anti-VEGF antibody or nonimmune IgG (A) was subjected to semiquantitative analysis (B), as described in the Materials and Methods section. VEGF expression was measured in sections of mouse placentas with the indicated genotype. P values of comparisons between Hbegf+/+ and Hbegf−/− in the labyrinth and spongiotrophoblast region (#, P = 0.06; **P < 0.01).

Article Snippet: Rehydrated sections of placenta were labeled for 1 h at 25°C with either 10 μg/ml nonimmune goat IgG (Jackson Immunoresearch Laboratories, West Grove, PA) or 5 μg/ml goat polyclonal antibody against human HBEGF (AF259), EGF (AF2028), TGFA (AF239), AREG (AF989), laminin (EMD Millipore AB2034), or vascular endothelial growth factor (VEGF) (AF293) (R&D systems unless otherwise indicated).

Techniques: Expressing, Immunohistochemical staining, Labeling